Tween 80 Biocompatible Surfactant for Suspension of Hydrophobic Particles, Microspheres and Density Marker Beads in Aqueous Media
Tween 80 Biocompatible Surfactant for Suspension of Hydrophobic Particles, Microspheres and Density Marker Beads in Aqueous Media

TWEEN 80 is a polyethylene sorbitol ester, also know as Polysorbate 80, PEG (80) sorbitan monooleate, polyoxyethylenesorbitan monooleate. TWEEN 80 is widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture, growing of tubercule bacilli, and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent except it has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may reduce the activity of many preservatives.

TWEEN 20 is a polyoxyethylene sorbitol esteris, a frequently used member of the polysorbate family. These have been used as emulsifying agents for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins). It has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.05 to 0.5%.

Please refer to the protocol for dispersing polyethylene microspheres in aqueous solutions.

For Laboratory Use Only. Not for drug, household or other uses.

TWEEN 80 is miscible in water (0.1 ml/ml) yielding a clear to slightly hazy faint yellow solution. It is reported to be miscible with alcohol, cottonseed oil, corn oil, ethyl acetate, methanol, and toluene, but insoluble in mineral oil. The pH of a 1% aqueous solution is 5.5-7.2.

TWEEN 20 is miscible in water (100 mg/ml), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate; and is practically insoluble in liquid paraffin and fixed oils.

The products are not sterile. Autoclaving of solutions is generally not advised. Sterile filtration is more easily done if the liquid is warmed to about 40°C and alternate portions of hot distilled water and TWEEN 80 are poured through the 0.22 μm filter. The TWEEN 80 will blend and remain in solution.

Select Particle Size and Density:
Item # Description/Size Status Price Qty
Tween 80 Biocompatible Surfactant - 2ml
In Stock $22.45
Tween 20 Biocompatible Surfactant - 2ml
In Stock $22.45
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Ask a Question about this Product:
If I wanted to make 1000 mL of a 0.05% water plus Tween 80 solution, what would be the formula? Thank you
For a 0.05% Tween solution you would use 0.5grams of Tween to 1000ml of DI water.
Cospheric Team August 9, 2020 12:54 PM