We appreciate your patience with any delays in shipment during pandemic.

My Account Shopping Cart Email Us Call Us
Home > FAQ and Resources > Research Using Cospheric Spheres > Research With PMMA Microspheres > Biophysical and Biochemical Markers of Red Blood Cell Fragility

Biophysical and Biochemical Markers of Red Blood Cell Fragility

Biophysical and Biochemical Markers of Red Blood Cell Fragility

Ariel Orbacha, Orly Zeligb, Saul Yedgara, Gregory Barshteina

(PMMA: 27-32um)


Abstract:

Background: Red blood cells (RBCs) undergo a natural aging process occurring in the blood circulation throughout the RBC lifespan or during routine cold storage in the blood bank. The aging of RBCs is associated with the elevation of mechanical fragility (MF) or osmotic fragility (OF) of RBCs, which can lead to cell lysis. The present study was undertaken to identify RBC properties that characterize their susceptibility to destruction under osmotic/mechanical stress.

Methods: RBCs were isolated from freshly donated blood or units of packed RBCs (PRBCs) and suspended in albumin-supplemented phosphate-buffered saline (PBS). In addition, PRBCs were separated by filtration through a microsphere column into two fractions: enriched with rigid (R-fraction) and deformable (D-fraction) cells. The RBCs were subjected to determination of deformability, MF and OF, moreover, the level of cell surface phosphatidylserine (PS) and the stomatin level in isolated RBC membranes were measured. Results: In the RBC population, the cells that were susceptible to mechanical and osmotic stress were characterized by low deformability and increased level of surface PS. The OF/MF was higher in the R-fraction than in the D-fraction. Stomatin was depleted in destroyed cells and in the R-fraction.

Conclusion: RBC deformability, the levels of surface PS, and membrane stomatin can be used as markers of RBC fragility.

Connect with us:

Facebook Twitter LinkedIn

We are here for you!

Do you have questions about our products? Need help with product selection?
Contact our technical support team at support@cospheric.com.

Sales inquiries?

Reach out to customer service at info@cospheric.com or +1-805-687-3747


Order online anytime

Check out online with a credit card or email a purchase order to info@cospheric.com

About the Company:

Cospheric is a global leader in manufacturing and distribution of precision nanospheres, microspheres, and larger precision spherical particles. Scientists and engineers worldwide rely on us as a proven source to fulfill their needs for microparticles in narrow size ranges, wide selection of colors, densities, properties and formulations. We leverage our extensive technical expertise in micropowders to help each customer find an optimal microparticle solution for their needs. We ship worldwide daily out of our Santa Barbara, CA manufacturing facility.
Terms and ConditionsPrivacy Policy
Cospheric LLC - the leading global supplier of precision spherical particles | Manufactured in Santa Barbara, CA, USA
Copyright © 2008-2020 Cospheric LLC. All Rights Reserved.